Zivancevic-Simonovic Snezana, Stojanovic Marijana, Inic-Kanada Aleksandra, Zivkovic Irena, Odrljin Tatjana, Dimitrijevic Ljiljana
Institute of Pathophysiology, School of Medicine, University of Kragujevac, Svetozara Markovica 69, 34 000 Kragujevac, Serbia and Montenegro.
In Vivo. 2006 Sep-Oct;20(5):621-7.
Network perturbations have been reported in autoimmune processes which could cause polyclonal cell activation.
Hyper-immunization was achieved by the injection of 3 mg of human monoclonal IgM DJ into BALB/c mice. Indirect ELISA was performed to detect induced antibody specificities. Competitive ELISAs were carried out on sera to detect the idiotypes expressed in first (Ab1) and second (Ab2) antibodies in sequence.
In the sera of hyper-immunized mice, the concentrations of Ab1 and Ab2 were respectively five and ten times higher than in the sera of normoimmunized mice. Polyclonal lymphocyte activation was demonstrated by an increase in antibodies specific for antigens (cytochrome C) not related to the immunizing antigen, elevated levels of IgM antibodies specific for the immunogen and elevated levels of Ab2s of the IgM isotype.
These findings are indicative of antigen-dependent network perturbation being a causative factor in polyclonal lymphocyte activation.
已有报道称自身免疫过程中存在网络扰动，这可能导致多克隆细胞活化。
通过向BALB/c小鼠注射3mg人单克隆IgM DJ实现超免疫。采用间接ELISA检测诱导抗体的特异性。对血清进行竞争性ELISA，依次检测第一抗体（Ab1）和第二抗体（Ab2）中表达的独特型。
在超免疫小鼠的血清中，Ab1和Ab2的浓度分别比正常免疫小鼠血清中的浓度高5倍和10倍。对与免疫抗原无关的抗原（细胞色素C）具有特异性的抗体增加、对免疫原具有特异性的IgM抗体水平升高以及IgM同种型的Ab2水平升高，证明了多克隆淋巴细胞活化。
这些发现表明抗原依赖性网络扰动是多克隆淋巴细胞活化的一个致病因素。
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