一种从RNA转录混合物中去除DNA模板的新型非酶法。
A novel non-enzymatic procedure for removing DNA template from RNA transcription mixtures.
作者信息
Kedzierski W, Porter J C
机构信息
University of Texas, Southwestern Medical Center.
出版信息
Biotechniques. 1991 Feb;10(2):210-4.
PMID:1711872
Abstract
A novel, simplified procedure for the removal of DNA template from newly synthesized RNA is described. The method avoids the use of DNase and involves extraction of the RNA transcription products at acid pH using either phenol or phenol: chloroform:guanidinium thiocyanate mixture. Both procedures are as effective as DNase digestion in removing DNA template, but are free of the hazard of RNA degradation, are highly reproducible and remove proteins at the same time.
摘要
本文描述了一种从新合成的RNA中去除DNA模板的新颖、简化方法。该方法避免使用DNA酶，而是在酸性pH条件下使用苯酚或苯酚：氯仿：硫氰酸胍混合物提取RNA转录产物。这两种方法在去除DNA模板方面与DNA酶消化一样有效，但不存在RNA降解的风险，具有高度的可重复性，并且能同时去除蛋白质。
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