Pruitt Kim D, Tatusova Tatiana, Maglott Donna R
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Rm 6An.12J, 45 Center Drive, Bethesda, MD 20892-6510, USA.
Nucleic Acids Res. 2007 Jan;35(Database issue):D61-5. doi: 10.1093/nar/gkl842. Epub 2006 Nov 27.
NCBI's reference sequence (RefSeq) database (http://www.ncbi.nlm.nih.gov/RefSeq/) is a curated non-redundant collection of sequences representing genomes, transcripts and proteins. The database includes 3774 organisms spanning prokaryotes, eukaryotes and viruses, and has records for 2,879,860 proteins (RefSeq release 19). RefSeq records integrate information from multiple sources, when additional data are available from those sources and therefore represent a current description of the sequence and its features. Annotations include coding regions, conserved domains, tRNAs, sequence tagged sites (STS), variation, references, gene and protein product names, and database cross-references. Sequence is reviewed and features are added using a combined approach of collaboration and other input from the scientific community, prediction, propagation from GenBank and curation by NCBI staff. The format of all RefSeq records is validated, and an increasing number of tests are being applied to evaluate the quality of sequence and annotation, especially in the context of complete genomic sequence.
美国国立医学图书馆国家生物技术信息中心（NCBI）的参考序列（RefSeq）数据库（http://www.ncbi.nlm.nih.gov/RefSeq/）是一个经过整理的非冗余序列集合，涵盖基因组、转录本和蛋白质。该数据库包含3774种生物，涵盖原核生物、真核生物和病毒，拥有2879860条蛋白质记录（RefSeq第19版）。RefSeq记录整合了来自多个来源的信息，当这些来源有额外数据时，因此代表了序列及其特征的当前描述。注释包括编码区、保守结构域、tRNA、序列标签位点（STS）、变异、参考文献、基因和蛋白质产物名称以及数据库交叉引用。序列经过审核，并使用合作及科学界其他输入、预测、从GenBank传播以及NCBI工作人员整理的组合方法添加特征。所有RefSeq记录的格式都经过验证，并且正在应用越来越多的测试来评估序列和注释的质量，特别是在完整基因组序列的背景下。
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