西尼罗河病毒的突变谱取决于宿主，并且是小鼠死亡率的一个决定因素。
The West Nile virus mutant spectrum is host-dependant and a determinant of mortality in mice.
作者信息
Jerzak Greta V S, Bernard Kristen, Kramer Laura D, Shi Pei-Yong, Ebel Gregory D
机构信息
The Arbovirus Laboratories, Wadsworth Center, New York State Department of Health, 5668 State Farm Rd., Slingerlands, NY 12159, USA.
出版信息
Virology. 2007 Apr 10;360(2):469-76. doi: 10.1016/j.virol.2006.10.029. Epub 2006 Nov 28.
To define the impact of mosquitoes and birds on intrahost WNV population dynamics, the mutant spectra that arose as a result of 20 serial in vivo passages in Culex pipiens and young chickens were examined. Genetically homogeneous WNV was serially passaged 20 times in each host. Genetic diversity was greater in mosquito-passaged WNV compared to chicken-passaged WNV. Changes in the viral consensus sequence occurred in WNV passaged in mosquitoes earlier and more frequently than in chicken-passaged WNV. Analysis of synonymous and nonsynonymous variation suggested that purifying selection was relaxed during passage in mosquitoes. Mortality in mice was significantly negatively correlated with the size of the WNV mutant spectrum. These studies suggest that mosquitoes serve as sources for WNV genetic diversity, that birds are selective sieves, and that both the consensus sequence and the mutant spectrum contribute to WNV phenotype.
为了确定蚊子和鸟类对宿主体内西尼罗河病毒（WNV）种群动态的影响，我们检测了在致倦库蚊和幼鸡体内连续传代20次后产生的突变谱。基因同质的WNV在每个宿主中连续传代20次。与经鸡传代的WNV相比，经蚊子传代的WNV遗传多样性更高。在蚊子体内传代的WNV中，病毒共有序列的变化比在鸡体内传代的WNV更早且更频繁地发生。同义变异和非同义变异分析表明，在蚊子体内传代期间纯化选择有所放松。小鼠死亡率与WNV突变谱的大小显著负相关。这些研究表明，蚊子是WNV遗传多样性的来源，鸟类是选择性筛子，并且共有序列和突变谱都对WNV表型有贡献。
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