阿霉素增强抗人DR5单克隆抗体（mDRA-6）诱导的HL-60细胞凋亡
[Adriamycin enhances anti-human DR5 monoclonal antibody (mDRA-6) induced HL-60 cells apoptosis].
作者信息
Li Shu-Lian, Ma Yuan-Fang, Liu Guang-Chao, Zhang Jun, Bai Hui-Ling, Liu Ying-Jie, Lu Feng
机构信息
Laboratory of Cellular and Molecular Immunology, Henan University, Kaifeng 475001, China.
出版信息
Zhonghua Xue Ye Xue Za Zhi. 2006 Jul;27(7):461-4.
OBJECTIVE
To investigate synergistic killing effect of anti-human DR5 (death receptor 5 of TRAIL) monoclonal antibody (mDRA-6) and adriamycin(Adr) on HL-60 cells.
METHODS
mDRA-6 was prepared by immunizing BALB/c mice with DR5 protein. DR5 expression on Adr-treated HL-60 cells was detected by flow cytometry. Morphologic changes of HL-60 cells were observed under fluorescence microscope. Cytotoxic and apoptotic effects of mDRA-6 and Adr on HL-60 cells were measured by MTT analysis. DNA fragmentation was detected by agarose gel electrophoresis.
RESULTS
Adr induce DR5 expression on HL-60 cells. Cell budding, chromatin condensation and apoptotic body formation were observed in HL-60 cells treated by mDRA-6 and Adr. Death and apoptosis of these cells and DNA ladder were exhibited on agarose gel electrophoresis.
CONCLUSION
mDRA-6 and Adr have synergistic killing effect on HL-60 cells.
目的
探讨抗人DR5（肿瘤坏死因子相关凋亡诱导配体的死亡受体5）单克隆抗体（mDRA-6）与阿霉素（Adr）对HL-60细胞的协同杀伤作用。
方法
用DR5蛋白免疫BALB/c小鼠制备mDRA-6。采用流式细胞术检测阿霉素处理的HL-60细胞上DR5的表达。在荧光显微镜下观察HL-60细胞的形态变化。采用MTT分析法检测mDRA-6和阿霉素对HL-60细胞的细胞毒性和凋亡作用。通过琼脂糖凝胶电泳检测DNA片段化。
结果
阿霉素诱导HL-60细胞上DR5的表达。在mDRA-6和阿霉素处理的HL-60细胞中观察到细胞出芽、染色质浓缩和凋亡小体形成。琼脂糖凝胶电泳显示这些细胞的死亡和凋亡以及DNA梯形条带。
结论
mDRA-6和阿霉素对HL-60细胞具有协同杀伤作用。
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