Zhao Jing, Zhou Jingmin, Liu Mingzhe, Asanuma Hiroyuki, Komiyama Makoto
Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1, Komaba, Meguro-ku, Tokyo 153-8904, Japan.
Nucleic Acids Symp Ser (Oxf). 2004(48):199-200. doi: 10.1093/nass/48.1.199.
Based on a previous study of photo-regulation of transcription by T7 RNA polymerase, the regulatory effect of azobenzene on in vitro green fluorescent protein (GFP) production was studied by introduction of a D-threoninol-tethered p-azobenzene into the non template strand of the T7 promoter in a GFP template. By measurement of the resulting GFP fluorescence intensity after the in vitro transcription/translation coupled reaction, it is found that photo-regulation of GFP gene expression can be achieved in a position-dependent manner by trans-cis isomerization of azobenzene upon UV irradiation.
基于先前对T7 RNA聚合酶转录光调节的研究，通过将D-苏糖醇连接的对-偶氮苯引入绿色荧光蛋白（GFP）模板中T7启动子的非模板链，研究了偶氮苯对体外绿色荧光蛋白产生的调节作用。通过测量体外转录/翻译偶联反应后产生的GFP荧光强度，发现通过紫外线照射后偶氮苯的反式-顺式异构化，可以以位置依赖的方式实现GFP基因表达的光调节。
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