通过具有2-甲基-1,4-萘醌发色团的光功能化寡脱氧核苷酸检测DNA中的5-甲基胞嘧啶。

Detection of 5-methylcytosine in DNA by photo-functionalized oligodeoxynucleotides possessing 2-methyl-1,4-naphthoquinone chromophore.

作者信息

Yamada Hisatsugu, Tanabe Kazuhito, Nishimoto Sei-ichi

机构信息

Department of Energy and Hydrocarbon Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Kyoto 615-8510, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2005(49):149-50. doi: 10.1093/nass/49.1.149.

DOI:10.1093/nass/49.1.149

PMID:17150677

Abstract

We developed a method for detection of 5-methylcytosine in DNA by photosensitized oxidation, using photo-functionalized oligodeoxynucleotides possessing 2-methyl-1,4-naphthoquinone chromophores (NQ) at the interior of the strand. The photooxidation and subsequent hot piperidine treatment of duplex consisting of the NQ-tethered oligodeoxynucleotide and its complementary strand bearing 5-methylcytosine residue led to selective DNA strand cleavage at the 5-methylcytosine residue, whereas no strand cleavage was detected for the control duplex with normal cytosine residue but not 5-methylcytosine.

摘要

我们开发了一种通过光敏氧化检测DNA中5-甲基胞嘧啶的方法，使用在链内部具有2-甲基-1,4-萘醌发色团（NQ）的光功能化寡脱氧核苷酸。对由NQ连接的寡脱氧核苷酸及其带有5-甲基胞嘧啶残基的互补链组成的双链体进行光氧化及随后的热哌啶处理，导致在5-甲基胞嘧啶残基处发生选择性DNA链断裂，而对于具有正常胞嘧啶残基而非5-甲基胞嘧啶的对照双链体未检测到链断裂。