由其笼蔽前体生成的C4'-氧化无碱基位点的胺修饰反应。

Amine modification reaction of C4'-oxidized abasic site generated from its caged precursors.

作者信息

Usui Kazuteru, Fukuda Mitsuhiro, Aso Mariko, Suemune Hiroshi

机构信息

Graduate School of pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2005(49):191-2. doi: 10.1093/nass/49.1.191.

DOI:10.1093/nass/49.1.191

PMID:17150698

Abstract

A method for the generation of ODNs containing a C4'-oxidized abasic site (C4'-OAS) from "photocaged" ODNs was investigated. We synthesized C4'-caged ODN 1 and C1'-caged ODN 2 bearing an o-nitrobenzyloxy group at the C-4' position and the C-1' position, respectively. The duplex stabilities of 1 and 2, with their complementary ODNs were studied by Tm measurements. The photochemical reaction of 1 and 2 to generate ODN 3 containing C4'-OAS, and the subsequent amine modification reaction were studied.

摘要

研究了一种从“光笼化”寡核苷酸（ODN）生成含有C4'-氧化脱碱基位点（C4'-OAS）的ODN的方法。我们合成了分别在C-4'位和C-1'位带有邻硝基苄氧基的C4'-笼化ODN 1和C1'-笼化ODN 2。通过测量熔解温度（Tm）研究了1和2与其互补ODN的双链稳定性。研究了1和2生成含有C4'-OAS的ODN 3的光化学反应以及随后的胺修饰反应。