利用链交换反应对DNA单碱基错配进行电化学检测。

Electrochemical detection of DNA single base mismatch by the use of strand exchange reaction.

作者信息

Kumamoto Satoshi, Maruyama Atsushi, Nakamura Mitsunobu, Yamana Kazushige

机构信息

Department of Materials Science and Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Syosya, Himeji 671-2201, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2006(50):93-4. doi: 10.1093/nass/nrl046.

DOI:10.1093/nass/nrl046

PMID:17150833

Abstract

The electrochemical method based on the strand exchange reaction (SER) for the detection of DNA single-base mismatch has been developed. Different electrochemical responses due to the slower SER rates for mismatch containing DNA than fully matched DNA were observed by using the redox-modified partial duplex DNA immobilized on gold electrode as an analytical probe.

摘要

基于链交换反应（SER）检测DNA单碱基错配的电化学方法已被开发出来。通过使用固定在金电极上的氧化还原修饰部分双链DNA作为分析探针，观察到由于含错配DNA的SER速率比完全匹配的DNA慢而产生的不同电化学响应。