Ahern M J, Kevat S, Hill W, Hayball P J, Harley H, Hall P D
Department of Medicine, Flinders University of South Australia.
Ann Rheum Dis. 1991 Jul;50(7):477-80. doi: 10.1136/ard.50.7.477.
Liver tissue from 16 patients with rheumatoid arthritis was studied. The patients had received low dose methotrexate weekly for a minimum of 12 months between two liver biopsies. The progression of pericellular fibrosis was measured by computerised image analysis. Extracts of these liver biopsy specimens were pooled into five samples according to the progression of hepatic fibrosis and analysed by high performance liquid chromatography. The concentrations of methotrexate, 2,4 diamino-N(10)-methylpteroic acid, and methotrexate polyglutamate were markedly increased in the samples obtained from the three patients who recorded the greatest increase in fibrosis. These preliminary data suggest that progression of hepatic fibrosis is related to the retention of methotrexate and metabolites in the liver.
对16例类风湿性关节炎患者的肝脏组织进行了研究。这些患者在两次肝脏活检期间每周接受低剂量甲氨蝶呤治疗至少12个月。通过计算机图像分析测量细胞周围纤维化的进展。根据肝纤维化的进展情况,将这些肝脏活检标本的提取物合并为五个样本,并通过高效液相色谱法进行分析。在纤维化增加最多的三名患者的样本中,甲氨蝶呤、2,4-二氨基-N(10)-甲基蝶酸和甲氨蝶呤聚谷氨酸的浓度显著增加。这些初步数据表明,肝纤维化的进展与甲氨蝶呤及其代谢产物在肝脏中的潴留有关。