人类细胞线粒体中多个拟核和DNA分子的组织

Organization of multiple nucleoids and DNA molecules in mitochondria of a human cell.

作者信息

Satoh M, Kuroiwa T

机构信息

University of Tsukuba, Institute of Biological Sciences, Ibaraki, Japan.

出版信息

Exp Cell Res. 1991 Sep;196(1):137-40. doi: 10.1016/0014-4827(91)90467-9.

DOI:10.1016/0014-4827(91)90467-9

PMID:1715276

Abstract

Mitochondrial nucleoids (mt-nucleoids) of the A2780 line of cultured human cells were stained with DAPI and observed using an epifluorescence microscope. The mt-nucleoids appeared to be organized compactly in mitochondria. Numbers of mt-nucleoids per mitochondrion ranged from 1 to more than 10, and 70% were "multinucleated" mitochondria. Intensities of fluorescence of mt-nucleoids in each mitochondrion were measured by a video-intensified microscope system (VIM system) and copy numbers of mitochondrial DNA (mtDNA) in each mitochondria were determined. The copy numbers of mtDNA per mitochondrion ranged from 1 to 15, and the average was 4.6. Because the cells had 107 mitochondria on average, the copy number of mtDNA per cell was estimated to be about 500.

摘要

用4',6-二脒基-2-苯基吲哚（DAPI）对培养的人A2780细胞系的线粒体类核（mt-类核）进行染色，并用落射荧光显微镜进行观察。mt-类核似乎在线粒体中紧密排列。每个线粒体中mt-类核的数量从1到10多个不等，70%是“多核”线粒体。通过视频增强显微镜系统（VIM系统）测量每个线粒体中mt-类核的荧光强度，并确定每个线粒体中线粒体DNA（mtDNA）的拷贝数。每个线粒体中mtDNA的拷贝数从1到15不等，平均为4.6。由于这些细胞平均有107个线粒体，因此估计每个细胞中mtDNA的拷贝数约为500。