鉴定人类免疫缺陷病毒1型的型别和毒株特异性表位的单克隆抗体的特性分析

Characterization of monoclonal antibodies identifying type and strain-specific epitopes of human immunodeficiency virus type 1.

作者信息

Robert V, Resnicoff M, Chermann J C, Devaux C

机构信息

INSERM U.322, Unité de Recherches sur les Rétrovirus et Maladies Associées, Marseille, France.

出版信息

Mol Cell Biochem. 1991 Apr 10;102(2):115-23. doi: 10.1007/BF00234569.

DOI:10.1007/BF00234569

PMID:1715507

Abstract

Several hybridoma cell lines were raised against the highly cytopathic Zaïrian isolate of Human Immunodeficiency Virus (HIV), HIV1-NDK. The specificity of the secreted monoclonal antibodies (mAb) was demonstrated by immunoblotting, radioimmunoprecipitation and immunofluorescence. Two hybridoma cell lines secreted mAb reacting with independent epitopes of the NDK p17 capsid protein and its precursors. One, RL16.24.5, is specific for the NDK isolate whereas the other, RL16.45.1, along with anti-p25 RL16.30.1 mAb, bind all HIV1 isolates but not HIV2. Together with the previously described mAb RL4.72.1 those reagents define lentivirus subfamily (HIV1, HIV2, SIV) type/subtype (HIV1) and strain (HIV1-NDK) specific epitopes expressed on HIV1-NDK core proteins. The last mAb RL16.76.1 binds the env gene products gp160 and gp120.

摘要

针对人类免疫缺陷病毒（HIV）的高度细胞病变型扎伊尔分离株HIV1-NDK，培育了几种杂交瘤细胞系。通过免疫印迹、放射免疫沉淀和免疫荧光证明了分泌的单克隆抗体（mAb）的特异性。两个杂交瘤细胞系分泌的单克隆抗体与NDK p17衣壳蛋白及其前体的独立表位发生反应。其中一个，RL16.24.5，对NDK分离株具有特异性，而另一个，RL16.45.1，与抗p25 RL16.30.1单克隆抗体一起，能结合所有HIV1分离株，但不结合HIV2。与先前描述的单克隆抗体RL4.72.1一起，这些试剂定义了慢病毒亚科（HIV1、HIV2、SIV）、类型/亚型（HIV1）和毒株（HIV1-NDK）在HIV核心蛋白上表达的特异性表位。最后一个单克隆抗体RL16.76.1结合env基因产物gp160和gp120。