前沿：KIR3DL1与HLA - A和HLA - B之间的等位基因特异性及肽依赖性相互作用
Cutting Edge: Allele-specific and peptide-dependent interactions between KIR3DL1 and HLA-A and HLA-B.
作者信息
Thananchai Hathairat, Gillespie Geraldine, Martin Maureen P, Bashirova Arman, Yawata Nobuyo, Yawata Makoto, Easterbrook Philippa, McVicar Daniel W, Maenaka Katsumi, Parham Peter, Carrington Mary, Dong Tao, Rowland-Jones Sarah
机构信息
Medical Research Council Human Immunology Unit, Weatherall Institute of Molecular Medicine, Oxford, United Kingdom.
出版信息
J Immunol. 2007 Jan 1;178(1):33-7. doi: 10.4049/jimmunol.178.1.33.
Although it is clear that KIR3DL1 recognizes Bw4(+) HLA-B, the role of Bw4(+) HLA-A allotypes as KIR3DL1 ligands is controversial. We therefore examined the binding of tetrameric HLA-A and -B complexes, including HLA2402, a common Bw4(+) HLA-A allotype, to KIR3DL1001, 005, 007, and 1502 allotypes. Only Bw4(+) tetramers bound KIR3DL1. Three of four HLA-A2402 tetramers bound one or more KIR3DL1 allotypes and all four KIR3DL1 allotypes bound to one or more HLA-A2402 tetramers, but with different binding specificities. Only KIR3DL1005 bound both HLA-A2402 and HLA-B5703 tetramers. HLA-A2402-expressing target cells were resistant to lysis by NK cells expressing KIR3DL1001 or 005. This study shows that HLA-A2402 is a ligand for KIR3DL1 and demonstrates how the binding of KIR3DL1 to Bw4(+) ligands depends upon the bound peptide as well as HLA and KIR3DL1 polymorphism.
虽然很明显KIR3DL1识别Bw4(+) HLA - B，但Bw4(+) HLA - A同种异型作为KIR3DL1配体的作用仍存在争议。因此，我们检测了包括常见的Bw4(+) HLA - A同种异型HLA2402在内的四聚体HLA - A和 - B复合物与KIR3DL1001、005、007和1502同种异型的结合情况。只有Bw4(+)四聚体与KIR3DL1结合。四个HLA - A2402四聚体中的三个与一种或多种KIR3DL1同种异型结合，并且所有四种KIR3DL1同种异型都与一种或多种HLA - A2402四聚体结合，但结合特异性不同。只有KIR3DL1005同时结合HLA - A2402和HLA - B5703四聚体。表达HLA - A2402的靶细胞对表达KIR3DL1001或005的NK细胞介导的裂解具有抗性。这项研究表明HLA - A2402是KIR3DL1的配体，并证明了KIR3DL1与Bw4(+)配体的结合如何取决于结合的肽以及HLA和KIR3DL1的多态性。
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