一种用于同时检测五种人类肝炎病毒（乙肝病毒、丙肝病毒、丁肝病毒、戊肝病毒和庚肝病毒）多种抗原和抗体的简单、快速且灵敏的集成蛋白质微阵列。

A simple, rapid, and sensitive integrated protein microarray for simultaneous detection of multiple antigens and antibodies of five human hepatitis viruses (HBV, HCV, HDV, HEV, and HGV).

作者信息

Xu Rongzhen, Gan Xiaoxian, Fang Yongming, Zheng Shu, Dong Qi

机构信息

Second Affiliated Hospital, Cancer Institute, School of Medicine, Zhejiang University, Hangzhou 310009, China.

出版信息

Anal Biochem. 2007 Mar 1;362(1):69-75. doi: 10.1016/j.ab.2006.12.008. Epub 2006 Dec 29.

DOI:10.1016/j.ab.2006.12.008

PMID:17239337

Abstract

Protein microarrays for parallel detection of multiple viral antigens and antibodies have not yet been described in the field of human hepatitis virus infections. Here, we describe a simple, rapid and sensitive integrated protein microarray with three different reaction models. The integrated protein microarray could simultaneously determine in human sera two viral antigens (HBsAg, HBeAg) and seven viral antibodies (HBsAb, HBcAb, HBeAb, HCVAb, HDVAb, HEVAb, HGVAb) of human hepatitis viruses within 20 min. The results of the protein microarray were assessed directly by the naked eye but can also be analyzed by a quantitative detector. The detection limit of this protein microarray was 0.1 ng/ml for HBsAg. Overall, >85% concordance was observed between the integrated protein microarrays and an enzyme-linked immunosorbent assay for above hepatitis viral antigen and antibody detections in human sera. This integrated protein microarray can be easily optimized for clinical use and epidemiological screening for multiple hepatitis virus infections.

摘要

用于并行检测多种病毒抗原和抗体的蛋白质微阵列在人类肝炎病毒感染领域尚未见报道。在此，我们描述了一种具有三种不同反应模式的简单、快速且灵敏的集成蛋白质微阵列。该集成蛋白质微阵列能够在20分钟内同时检测人血清中两种人类肝炎病毒的病毒抗原（HBsAg、HBeAg）和七种病毒抗体（HBsAb、HBcAb、HBeAb、HCVAb、HDVAb、HEVAb、HGVAb）。蛋白质微阵列的结果可直接通过肉眼评估，也可通过定量检测仪进行分析。该蛋白质微阵列对HBsAg的检测限为0.1 ng/ml。总体而言，在检测人血清中上述肝炎病毒抗原和抗体时，集成蛋白质微阵列与酶联免疫吸附测定法之间的一致性超过85%。这种集成蛋白质微阵列可轻松优化用于临床应用和多种肝炎病毒感染的流行病学筛查。