内皮素-3在体外直接影响大鼠下丘脑腹侧第三脑室区域和视上核的神经元。

Endothelin-3 directly affects neurons in the anteroventral third ventricle region and supraoptic nucleus of the rat hypothalamus in vitro.

作者信息

Yamashita H, Yamamoto S, Inenaga K, Kannan H

机构信息

First Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.

出版信息

J Cardiovasc Pharmacol. 1991;17 Suppl 7:S200-2. doi: 10.1097/00005344-199100177-00057.

DOI:10.1097/00005344-199100177-00057

PMID:1725332

Abstract

Extracellular recordings were made from anteroventral third ventricle (AV3V) and supraoptic nucleus (SON) neurons of rat hypothalamus in slice preparations. ET-3 was applied at concentrations of 10(-10) to 3 x 10(-7) M. Of 119 AV3V neurons tested, 21 (18%) were excited, 8 (6%) were slightly inhibited, and 90 (76%) were unaffected. The threshold concentration to evoke responses was approximately 10(-8) M. Excitatory responses of the AV3V neurons to ET-3 remained in a Ca(2+)-free medium. Of 120 SON neurons tested, 46 were phasic (putative vasopressin neuron) and 74 were nonphasic (putative oxytocin neuron). Of 46 phasic neurons tested, 26 (56.5%) were inhibited by ET-3, 20 (43.5%) were nonresponsive, and none was excited. Of 74 nonphasic neurons tested, 14 (19%) neurons were inhibited by ET-3, 60 (81%) were nonresponsive, and none was excited. These results suggest that ET-3 has dual effects on AV3V and SON neurons and the mechanisms of the responses may be different in the AV3V and SON neurons.

摘要

在脑片标本中，从大鼠下丘脑的腹前第三脑室（AV3V）和视上核（SON）神经元进行细胞外记录。以10（-10）至3×10（-7）M的浓度应用ET-3。在119个被测试的AV3V神经元中，21个（18%）被兴奋，8个（6%）受到轻微抑制，90个（76%）未受影响。诱发反应的阈值浓度约为10（-8）M。AV3V神经元对ET-3的兴奋反应在无钙培养基中依然存在。在120个被测试的SON神经元中，46个为相位性（推测为血管加压素神经元），74个为非相位性（推测为催产素神经元）。在46个被测试的相位性神经元中，26个（56.5%）被ET-3抑制，20个（43.5%）无反应，无一个被兴奋。在74个被测试的非相位性神经元中，14个（19%）被ET-3抑制，60个（81%）无反应，无一个被兴奋。这些结果表明，ET-3对AV3V和SON神经元具有双重作用，且在AV3V和SON神经元中的反应机制可能不同。