一组用于牙鲆（Paralichthys olivaceus）的多态性三核苷酸和四核苷酸微卫星标记。

A set of polymorphic trinucleotide and tetranucleotide microsatellite markers for the Japanese flounder (Paralichthys olivaceus).

作者信息

Castaño-Sanchez C, Fuji K, Hayashida K, Tagami M, Ozaki A, Hasegawa O, Sakamoto T, Kawai J, Hayashizaki Y, Okamoto N

机构信息

Tokyo University of Marine Science and Technology, Minato, Tokyo, Japan.

出版信息

Anim Genet. 2007 Feb;38(1):75-6. doi: 10.1111/j.1365-2052.2006.01548.x.

DOI:10.1111/j.1365-2052.2006.01548.x

PMID:17257193

Abstract

We have developed the first set of trinucleotide and tetranucleotide markers for the Japanese flounder, Paralichthys olivaceus. One hundred and sixty-seven polymorphic trinucleotide and tetranucleotide microsatellites were isolated using clones derived from two libraries. Of almost 200,000 clones analysed, 0.5% presented trinucleotide or tetranucleotide repeat regions. Among the trinucleotide repeats analysed in this study, the most frequent one was (CAG)(n) and the most common tetranucleotide repeat was (GATA)(n). The position of the new markers in the genetic linkage map was determined. Markers were evenly distributed along the P. olivaceus linkage groups, without distinction between the kinds of repeats and library of origin. The markers isolated in this study contribute significantly to the genetic linkage map of the Japanese flounder.

摘要

我们开发了第一组用于牙鲆（Paralichthys olivaceus）的三核苷酸和四核苷酸标记。利用来自两个文库的克隆分离出了167个多态性三核苷酸和四核苷酸微卫星。在分析的近200,000个克隆中，0.5%呈现出三核苷酸或四核苷酸重复区域。在本研究分析的三核苷酸重复中，最常见的是(CAG)(n)，最常见的四核苷酸重复是(GATA)(n)。确定了新标记在遗传连锁图谱中的位置。标记沿着牙鲆连锁群均匀分布，与重复类型和来源文库无关。本研究中分离出的标记对牙鲆的遗传连锁图谱有显著贡献。