菊花中主要黄酮苷的测定及含量影响因素观察
[Determination of main flavone glycosides in Flos Chrysanthemi and observation of factors influenced contents].
作者信息
Hu Bi-Bo, Wu Zu-Shuai, Cai Jun, Jiang Hui-Di
机构信息
Department of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2006 Nov;31(21):1772-5.
OBJECTIVE
To determine and compare the content of luteolin-7-O-beta-D-glucoside and apigenin-7-O-beta-D-glucoside in Flos Chrysanthemi from different collection time, sources, grades and processes.
METHOD
The contents were determined by RP-HPLC. Zorbax SB C18 column (4.6 mm x 250 mm, 5 microm) was used as analysis column, the mobile phase was acetonitrile-pH 2.0 phosphate buffer solution with gradient elution, the detector was set at 338 nm.
RESULT
The contents of two components changed at some degree in Flos Chrysanthemi from different collection time, different plant sites or with different grades, while the contents varied obviously among Flos Chrysanthemi from different source and different sorts. No obvious difference was found in Flos Chrysanthemi from different year.
CONCLUSION
The contents of two components were influenced by process, plane site, source and sorts, especially by source and sorts.
目的
测定并比较不同采收时间、来源、等级及加工方法的菊花中木犀草素 -7-O-β-D-葡萄糖苷和芹菜素 -7-O-β-D-葡萄糖苷的含量。
方法
采用反相高效液相色谱法测定含量。以Zorbax SB C18柱（4.6 mm×250 mm，5 µm）为分析柱，流动相为乙腈 - pH 2.0磷酸盐缓冲溶液，梯度洗脱，检测波长为338 nm。
结果
不同采收时间、不同产地或不同等级的菊花中两种成分的含量有一定变化，而不同来源和不同品种的菊花中含量差异明显。不同年份的菊花中未发现明显差异。
结论
两种成分的含量受加工方法、产地、来源和品种的影响，尤其受来源和品种的影响。
Zotero 插件