米曲霉植酸酶基因在米曲霉RIB40 niaD(-)中的表达。
Expression of Aspergillus oryzae phytase gene in Aspergillus oryzae RIB40 niaD(-).
作者信息
Uchida Hiroyuki, Arakida Shinya, Sakamoto Tatsuji, Kawasaki Haruhiko
机构信息
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, University of Fukui, 9-1 Bunkyo 3-chome, Fukui-shi 910-8507, Japan.
出版信息
J Biosci Bioeng. 2006 Dec;102(6):564-7. doi: 10.1263/jbb.102.564.
PMID:17270723
Abstract
Aspergillus oryzae RIB40 niaD(-) was transformed using a plasmid constructed with the A. oryzae phytase gene and pNAN8142 vector. The culture broth of the transformant, which was grown in a medium containing starch as a carbon source and polyvinylpyrrolidone showed phytase activity of a maximum of 2.0 units ml(-1) at 37 degrees C, pH 5.5.
摘要
使用由米曲霉植酸酶基因和pNAN8142载体构建的质粒对米曲霉RIB40 niaD(-)进行转化。该转化体在以淀粉作为碳源和聚乙烯吡咯烷酮的培养基中培养，其培养液在37℃、pH 5.5条件下的植酸酶活性最高可达2.0单位/毫升。
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