用于检测创伤弧菌生物型2及同时鉴别血清型E菌株的多重聚合酶链反应检测法。
Multiplex PCR assay for detection of Vibrio vulnificus biotype 2 and simultaneous discrimination of serovar E strains.
作者信息
Sanjuán Eva, Amaro Carmen
机构信息
Departamento Microbiología y Ecología, Universidad de Valencia, Avenida Dr. Moliner 50, 46100 Burjassot, Valencia, Spain.
出版信息
Appl Environ Microbiol. 2007 Mar;73(6):2029-32. doi: 10.1128/AEM.02320-06. Epub 2007 Feb 2.
Abstract
In the present work we develop a multiplex PCR assay for the detection and identification of the fish pathogen Vibrio vulnificus biotype 2 with discriminating potential for zoonotic strains (serovar E). The PCR assay allowed the identification of two new biotype 2 serovar E human isolates from culture collections. Finally, the multiplex was successfully applied to both diagnosis and carrier detection in field samples.
摘要
在本研究中，我们开发了一种多重PCR检测方法，用于检测和鉴定鱼类病原体创伤弧菌生物型2，该方法对人畜共患病菌株（血清型E）具有鉴别潜力。该PCR检测方法能够从培养物保藏中心鉴定出两株新的生物型2血清型E人类分离株。最后，该多重PCR检测方法成功应用于现场样本的诊断和携带者检测。
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