一种抗炭疽芽孢杆菌亚单位疫苗的免疫原性。
Immunogenicity of a subunit vaccine against Bacillus anthracis.
作者信息
Chichester Jessica A, Musiychuk Konstantin, de la Rosa Patricia, Horsey April, Stevenson Natalie, Ugulava Natalia, Rabindran Shailaja, Palmer Gene A, Mett Vadim, Yusibov Vidadi
机构信息
Fraunhofer USA Center for Molecular Biotechnology, 9 Innovation Way, Suite 200, Newark DE 19711, USA.
出版信息
Vaccine. 2007 Apr 20;25(16):3111-4. doi: 10.1016/j.vaccine.2007.01.068. Epub 2007 Jan 22.
The current approved vaccine against anthrax is based on protective antigen (PA) of Bacillus anthracis, requires six injections over an 18-month period and has a known history of side effects. Therefore, there is significant effort towards developing an improved vaccine against B. anthracis. Here we separately engineered and expressed domain 4 of PA (PAD4) and domain 1 of lethal factor (LFD1) as fusions to lichenase (LicKM), a thermostable enzyme from Clostridium thermocellum, and transiently expressed these fusions in Nicotiana benthamiana. Plant-produced antigens were combined and immunogenicity was evaluated in mice. All animals that received the experimental vaccine developed high antibody titers that were predominantly IgG1 and were able to neutralize the effects of LeTx in vitro.
目前批准的炭疽疫苗是基于炭疽芽孢杆菌的保护性抗原（PA），需要在18个月内进行六次注射，并且有已知的副作用史。因此，人们在大力研发一种改进的炭疽芽孢杆菌疫苗。在这里，我们分别构建并表达了PA的结构域4（PAD4）和致死因子的结构域1（LFD1），并将它们与来自嗜热栖热梭菌的一种耐热酶地衣酶（LicKM）融合，然后在本氏烟草中瞬时表达这些融合蛋白。将植物产生的抗原组合起来，并在小鼠中评估其免疫原性。所有接受实验性疫苗的动物都产生了高抗体滴度，主要为IgG1，并且能够在体外中和致死毒素（LeTx）的作用。
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