[Differential analysis of two-dimensional gel electrophoresis profiles of spermatozoal protein in human normal semen and idiopathic asthenospermia].

OBJECTIVES

To evaluate the application of two-dimensional electrophoresis in the research of differentially expressed proteins in human spermatozoa.

METHODS

Two-dimensional gel electrophoresis was performed on 4 sperm samples from normal healthy men and another 4 from asthenospermia patients. After silver staining, the differential expression proteins were analyzed by PDQuest 2D analysis software.

RESULTS

Seven differential protein spots were identified, 2 expressed highly in the asthenospermia sperm but lowly in the normal spermatozoa, while the other 5 expressed just the opposite way.

CONCLUSION

The protein profiles of differential expression between the normal spermatozoa and idiopathic asthenospermia were established and some differential proteins were found. The data obtained in this study may help prepare the ground for further studies on the isolation and identification of differentially expressed proteins in human asthenospermia sperm.