R6K质粒在大肠杆菌细胞周期中的复制。
Replication of the R6K plasmid during the Escherichia coli cell cycle.
作者信息
Keasling J D, Palsson B O, Cooper S
机构信息
Department of Chemical Engineering, University of Michigan, Ann Arbor 48109-2136.
出版信息
J Bacteriol. 1992 Feb;174(3):1060-2. doi: 10.1128/jb.174.3.1060-1062.1992.
Abstract
The cell-cycle replication pattern of the R6K plasmid has been investigated by using the membrane-elution technique to produce cells labelled at different times during the division cycle and scintillation counting for quantitative analysis of radioactive plasmid DNA. The high-copy plasmid R6K replicates exponentially in a cell-cycle-independent manner. A mini-R6K plasmid deleted for the ori alpha origin of replication also replicates, exponentially in a cell-cycle-independent manner.
摘要
通过使用膜洗脱技术来培养在分裂周期不同时间点被标记的细胞，并采用闪烁计数法对放射性质粒DNA进行定量分析，研究了R6K质粒的细胞周期复制模式。高拷贝质粒R6K以与细胞周期无关的方式呈指数复制。缺失oriα复制起点的微型R6K质粒也以与细胞周期无关的方式呈指数复制。
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本文引用的文献
1
The nucleotide sequence of the replication origin beta of the plasmid R6K.
J Biol Chem. 1982 Nov 25;257(22):13823-7.
2
Replication properties of plasmic R6K.
Plasmid. 1981 Jan;5(1):2-9. doi: 10.1016/0147-619x(81)90073-1.
3
Three origins of replication are active in vivo in the R plasmid RSF1040.
J Biol Chem. 1980 Dec 10;255(23):11075-7.
4
Circular R-factor molecules controlling penicillinase synthesis, replicating in Escherichia coli under either relaxed or stringent control.
J Bacteriol. 1970 Oct;104(1):34-44. doi: 10.1128/jb.104.1.34-44.1970.
5
DNA synthesis during the division cycle of rapidly growing Escherichia coli B/r.
J Mol Biol. 1968 Feb 14;31(3):507-18. doi: 10.1016/0022-2836(68)90424-5.
6
Pattern of replication of a colicin factor during the cell cycle of Escherichia coli.
J Bacteriol. 1972 Dec;112(3):1425-7. doi: 10.1128/jb.112.3.1425-1427.1972.
7
Cell cycle-specific replication of Escherichia coli minichromosomes.
Proc Natl Acad Sci U S A. 1986 Jul;83(14):5101-5. doi: 10.1073/pnas.83.14.5101.
8
Replication patterns of multiple plasmids coexisting in Escherichia coli.
J Bacteriol. 1988 Mar;170(3):1380-3. doi: 10.1128/jb.170.3.1380-1383.1988.
9
A replication initiator protein enhances the rate of hybrid formation between a silencer RNA and an activator RNA.
Cell. 1987 Nov 6;51(3):455-62. doi: 10.1016/0092-8674(87)90641-6.
10
Cell-cycle-specific F plasmid replication: regulation by cell size control of initiation.
J Bacteriol. 1991 Apr;173(8):2673-80. doi: 10.1128/jb.173.8.2673-2680.1991.
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