沙特患者中幽门螺杆菌cagA和iceA基因型状态与消化性溃疡风险

Helicobacter pylori cagA and iceA genotypes status and risk of peptic ulcer in Saudi patients.

作者信息

Momenah Aiman M, Tayeb Mohammed T

机构信息

Department of Medical Microbiology, Faculty of Medicine, University of Umm Al-Qura, Makkah, Kingdom of Saudi Arabia.

出版信息

Saudi Med J. 2007 Mar;28(3):382-5.

PMID:17334464

Abstract

OBJECTIVE

To determine the prevalence of cagA+ and iceA genotypes among Helicobacter pylori (H. pylori) isolates from a group of Saudi patients with gastric complaints, and to find out any significant correlation between these strains and severe gastric clinical outcomes such as peptic ulcer and gastric cancer in Saudi population.

METHODS

A total of 1104 gastric biopsies from 368 patients who presented with symptoms suggestive of chronic gastritis, peptic ulcer disease, or gastric carcinoma were taken from the main hospitals in the Western region of Saudi Arabia from July 2004 to July 2005. We cultured the samples for H. pylori and a polymerase chain reaction was carried out to check for the presence or absence of cagA gene and the status of iceA genotypes.

RESULTS

Among the 368 suspected patients to be infected with H. pylori by means of clinical features and endoscopic findings; 103 (28%) were positive using culture technique. The relation of the presence of cagA and the development of cases to gastritis and ulcer was statistically significant (p=0.0001). Furthermore, this study revealed that 100% of ulcer cases were infected with iceA1 with a statistically significant correlation (p=0.0001), while 94.6% of gastritis and 90.9% of normal were infected with iceA2 (p=0.0001). Moreover cagA+/iceA1 combined genotypes was statistically correlated with peptic ulcer (100%) but not cagA-/iceA1 (0%; p=0.0001).

CONCLUSION

Certain H. pylori genotypes were more virulent than others. Multiple clinical implications based on these finding might be studied further.

摘要

目的

确定一组有胃部不适症状的沙特患者中幽门螺杆菌（H. pylori）分离株的cagA+和iceA基因型的流行情况，并找出这些菌株与沙特人群中消化性溃疡和胃癌等严重胃部临床结局之间是否存在显著相关性。

方法

2004年7月至2005年7月，从沙特阿拉伯西部地区的主要医院采集了368例表现出慢性胃炎、消化性溃疡疾病或胃癌症状的患者的1104份胃活检样本。我们对样本进行幽门螺杆菌培养，并进行聚合酶链反应以检查cagA基因的有无及iceA基因型的状态。

结果

在368例根据临床特征和内镜检查结果怀疑感染幽门螺杆菌的患者中，采用培养技术有103例（28%）呈阳性。cagA的存在与胃炎和溃疡病例的发生之间的关系具有统计学意义（p = 0.0001）。此外，本研究显示100%的溃疡病例感染了iceA1，具有统计学显著相关性（p = 0.0001），而94.6%的胃炎患者和90.9%的正常人均感染了iceA2（p = 0.0001）。此外，cagA+/iceA1联合基因型与消化性溃疡在统计学上相关（100%），而cagA-/iceA1则无相关性（0%；p = 0.0001）。