Januchowski R, Jagodziński P P
Department of Biochemistry and Molecular Biology, University of Medical Sciences, ul. Swiecickiego 6, 60-781 Poznań, Poland.
Adv Med Sci. 2006;51:178-80.
Deficient CD3-zeta chain expression in T cells of patients with idiopathic SLE is associated with T cell receptor/ /CD3 complex (TCR/CD3)-mediated signaling defect. Hydralazine (HYD) inhibits expression of DNA methyltransferase 1 (DNMT1) and may cause a lupus-like disease in man.
To explain the HYD effect on intracellular level of CD3-zeta chain in Jurkat T leukemia cells clone E6-1, we employed the flow cytometric analysis.
We observed a dose-dependent increase in cellular content of CD3-zeta chain in Jurkat T cells treated with HYD. Our results suggest that HYD may result in T cells dysfunction different from this observed in idiopathic SLE T cells.
This difference may partially explain distinct disease course in patients with HYD induced and idiopathic SLE.
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