Durso Lisa M, Bono James L, Keen James E
USDA, ARS, U.S. Meat Animal Research Center, P.O. Box 166, State Spur 18D, Clay Center, Nebraska 68933, USA.
J Microbiol Methods. 2007 May;69(2):381-3. doi: 10.1016/j.mimet.2007.01.016. Epub 2007 Feb 7.
Accurate Escherichia coli serotyping is critical for pathogen diagnosis and surveillance of non-O157 Shiga-toxigenic strains, however few laboratories have this capacity. The molecular serotyping protocol described in this paper targets the somatic and flagellar antigens of E. coli O111:H8 used in traditional serotyping, and can be performed routinely in the laboratory.
准确的大肠杆菌血清型鉴定对于非O157产志贺毒素菌株的病原体诊断和监测至关重要,然而很少有实验室具备这种能力。本文所述的分子血清型鉴定方案针对传统血清型鉴定中使用的大肠杆菌O111:H8的菌体抗原和鞭毛抗原,并且可以在实验室中常规进行。