陡脉冲电场通过改变细胞内钙浓度来调节细胞凋亡。

Steep pulsed electric fields modulate cell apoptosis through the change of intracellular calcium concentration.

作者信息

Tang Li-Ling, Sun Cai Xin, Liu Huan, Mi Yan, Yao Chen-Guo, Li Cheng-Xiang

机构信息

The Key Laboratory of High Voltage Engineering and Electrical New Technology, Ministry of Education, Chongqing, China.

出版信息

Colloids Surf B Biointerfaces. 2007 Jun 15;57(2):209-14. doi: 10.1016/j.colsurfb.2007.02.008. Epub 2007 Feb 11.

DOI:10.1016/j.colsurfb.2007.02.008

PMID:17376660

Abstract

A steep electric pulsed field with low intensity (150-250V/cm) and relative long time (10 min) was applied to adherent liver cancer cell line SMMC-7721 and the liver cell line HL-7702. Results showed that the electric field with intensity of 200 and 250V/cm could trigger cell apoptosis, whereas the SMMC-7721 cell was more sensitive to the electric stimulation than the HL-7702 cell. Laser Scanning Confocal Microscope (LSCM) was used to measuring the real-time change of cytosolic free Ca(2+) concentration. When cells were exposed electric pulses with 100V/cm intensity for 10 min, there was no significant change of intracellular calcium concentration. With the intensity increased to 200 and 250V/cm, intracellular calcium concentration decreased significantly. Results demonstrated the relationship between the apoptosis and change of intracellular calcium concentration. And the steep electric pulsed field can be used to the cancer therapy.

摘要

将强度较低（150 - 250V/cm）且时间相对较长（10分钟）的陡峭电脉冲场施加于贴壁肝癌细胞系SMMC - 7721和肝细胞系HL - 7702上。结果表明强度为200和250V/cm的电场可引发细胞凋亡，而SMMC - 7721细胞比HL - 7702细胞对电刺激更敏感。使用激光扫描共聚焦显微镜（LSCM）来测量胞质游离Ca(2+)浓度的实时变化。当细胞暴露于强度为100V/cm的电脉冲10分钟时，细胞内钙浓度无显著变化。随着强度增加到200和250V/cm，细胞内钙浓度显著降低。结果证明了细胞凋亡与细胞内钙浓度变化之间存在关联。并且陡峭电脉冲场可用于癌症治疗。