抗青光眼药物在培养的角膜内皮细胞中的细胞毒性。
Cellular cytotoxicity of antiglaucoma drugs in cultured corneal endothelial cells.
作者信息
Wu Kwou-Yeung, Wang Hwei-Zu, Hong Show-Jen
机构信息
Department of Ophthalmology, \College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
出版信息
Kaohsiung J Med Sci. 2007 Mar;23(3):105-11. doi: 10.1016/S1607-551X(09)70384-5.
In this study, the various antiglaucoma drugs including betaxolol, timolol, levobunolol, carteolol, brimonidine, dipivefrin, dorzolamide, brinzolamide, latanoprost, unoprostone, and pilocarpine were used to investigate the effects of cellular cytotoxicity in cultured bovine corneal endothelial cells. After exposure to the drugs in three dilutions, 1/100, 1/1,000, and 1/10,000, for 100 minutes, cells were estimated based on the release assay of lactate dehydrogenase (LDH) enzyme. It was found that cellular LDH was significantly released in the medium only at 1/100th dilution of betaxolol, brimonidine, dorzolamide, dipivefrin, latanoprost and unoprostone to 130%, 123%, 145%, 157%, 128% and 237%, respectively, compared with controls upon exposure to drugs for 100 minutes. Moreover, benzalkonium chloride preservative at the concentrations ranging from 0.001 to 0.00001 mg/mL did not affect cellular LDH release in bovine corneal endothelial cells. These results indicate that high concentrations of antiglaucoma drugs may induce cytotoxicity in corneal endothelial cells.
在本研究中，使用了多种抗青光眼药物，包括倍他洛尔、噻吗洛尔、左布诺洛尔、卡替洛尔、溴莫尼定、地匹福林、多佐胺、布林佐胺、拉坦前列素、乌诺前列酮和毛果芸香碱，来研究它们对培养的牛角膜内皮细胞的细胞毒性作用。在将药物以1/100、1/1000和1/10000三种稀释度暴露100分钟后，根据乳酸脱氢酶（LDH）的释放试验对细胞进行评估。结果发现，仅在倍他洛尔、溴莫尼定、多佐胺、地匹福林、拉坦前列素和乌诺前列酮以1/100稀释度暴露100分钟后，培养基中的细胞LDH显著释放，分别比对照组增加到130%、123%、145%、157%、128%和237%。此外，浓度范围为0.001至0.00001 mg/mL的苯扎氯铵防腐剂对牛角膜内皮细胞的细胞LDH释放没有影响。这些结果表明，高浓度的抗青光眼药物可能会诱导角膜内皮细胞的细胞毒性。
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