用于调节真核细胞生长的人肠道微生物组宏基因组克隆的高通量表型分析技术的开发。
Development of high-throughput phenotyping of metagenomic clones from the human gut microbiome for modulation of eukaryotic cell growth.
作者信息
Gloux Karine, Leclerc Marion, Iliozer Harout, L'Haridon René, Manichanh Chaysavanh, Corthier Gérard, Nalin Renaud, Blottière Hervé M, Doré Joël
机构信息
Unité d'Ecologie et de Physiologie du Système Digestif, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France.
出版信息
Appl Environ Microbiol. 2007 Jun;73(11):3734-7. doi: 10.1128/AEM.02204-06. Epub 2007 Mar 30.
Abstract
Metagenomic libraries derived from human intestinal microbiota (20,725 clones) were screened for epithelial cell growth modulation. Modulatory clones belonging to the four phyla represented among the metagenomic libraries were identified (hit rate, 0.04 to 8.7% depending on the screening cutoff). Several candidate loci were identified by transposon mutagenesis and subcloning.
摘要
对源自人类肠道微生物群的宏基因组文库（20,725个克隆）进行上皮细胞生长调节筛选。鉴定出属于宏基因组文库中所代表的四个门的调节性克隆（命中率，根据筛选阈值为0.04%至8.7%）。通过转座子诱变和亚克隆鉴定出了几个候选基因座。
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