麦角菌属产麦角胺菌株的颗粒组分将麦角棒麦角碱转化为巴斯帕酸

Conversion of Elymoclavine to Paspalic Acid by a Particulate Fraction from an Ergotamine-Producing Strain of Claviceps sp.

作者信息

Kim S U, Cho Y J, Floss H G, Anderson J A

机构信息

Department of Chemistry, Texas Tech University, Lubbock, TX 79409.

出版信息

Planta Med. 1983 Jul;48(7):145-8. doi: 10.1055/s-2007-969910.

DOI:10.1055/s-2007-969910

PMID:17404974

Abstract

The particulate fraction from the ergotamine-producing strain CLAVICEPS sp. PCCE1 catalyzed the conversion of [ (14)C]elymoclavine to paspalic acid. NADPH was required. Maximum conversion was 95%. Carbon monoxide (CO:0 (2), 4:1) and SK&F 525A (1.0 mM), cytochrome P-450 inhibitors, inhibited the conversion 94% and 50%, respectively. Minor amounts of paspalic acid (0.1 mg/1) were present in cultures. The particulate fraction from CLAVICEPS sp. SD 58, which accumulates elymoclavine in cultures, lacked activity for the conversion of elymoclavine to paspalic acid.

摘要

来自产麦角胺菌株麦角菌属PCCE1的微粒体部分催化了[(14)C]野麦角碱向巴斯帕酸的转化。需要NADPH。最大转化率为95%。一氧化碳（CO:0(2)，4:1）和SK&F 525A（1.0 mM），细胞色素P-450抑制剂，分别抑制转化率94%和50%。培养物中存在少量巴斯帕酸（0.1 mg/1）。来自麦角菌属SD 58的微粒体部分，其在培养物中积累野麦角碱，缺乏将野麦角碱转化为巴斯帕酸的活性。