Yao Jing, Shi Ya-Qin, Li Zhuo-Rong, Jin Shao-Hong
Institute of Medicinal Biotechnology, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100050, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jun 15;853(1-2):254-9. doi: 10.1016/j.jchromb.2007.03.022. Epub 2007 Mar 25.
Pharmaceutical counterfeiting is becoming a serious problem in the world, especially in developing countries including China. Herein an isocratic reversed-phase high performance liquid chromatography (RP-HPLC) method was developed for screening counterfeit medicines and adulterated dietary supplement products. The developed method could be employed to separate and determine simultaneously six anti-diabetic drugs (glipizide, gliclazide, glibenclamide, glimepiride, gliquidone, repaglinide) on an isocratic solvent system using an Alltima C18 column (5 microm, 150 mmx4.6 mm) with an isocratic mobile phase of methanol-phosphate buffer (pH 3.0; 0.01 mol/L) (70:30, v/v), at a flow rate of 1.0 mL/min and at a wavelength of 230 nm. The proposed method was successfully applied to the analysis of medicinal and dietary supplement samples purchased from the local market in China.
药品造假在全球正成为一个严重问题，在中国等发展中国家尤为如此。在此，开发了一种等度反相高效液相色谱（RP-HPLC）方法用于筛查假药和掺假的膳食补充剂产品。所开发的方法可用于在等度溶剂系统中，使用Alltima C18柱（5微米，150毫米×4.6毫米），以甲醇-磷酸盐缓冲液（pH 3.0；0.01摩尔/升）（70:30，v/v）作为等度流动相，流速为1.0毫升/分钟，波长为230纳米，同时分离和测定六种抗糖尿病药物（格列吡嗪、格列齐特；格列本脲、格列美脲、格列喹酮、瑞格列奈）。该方法成功应用于对从中国当地市场购买的药品和膳食补充剂样品的分析。
