Chen Chu, Zhang Hao, Xiao Wei, Yong Zheng-Ping, Bai Nan
West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
J Chromatogr A. 2007 Jun 22;1154(1-2):250-9. doi: 10.1016/j.chroma.2007.03.097. Epub 2007 Mar 30.
Using high-performance liquid chromatography (HPLC), a chemical fingerprint method was developed for investigating and demonstrating the variance of flavonoids among different origins of sea buckthorn berries. Thirty-four samples were analyzed including 15 RS (Hippophae rhamnoides ssp. sinensis) samples, 7 RY (H. rhamnoindes ssp. yunnanensis) samples, 5 RW (H. rhamnoides ssp. wolongensis) samples, 4 NS (H. neurocarpa ssp. stellatopilosa) samples and 3 TI (H. tibetana) samples. In the HPLC chromatograms, 12 compounds were identified as flavonoids, including quercetin 3-O-sophoroside-7-rhamnoside, kaempferol 3-O-sophoroside-7-O-rhamnoside, isorhamnetin 3-O-sophoroside-7-O-rhamnoside, isorhamnetin 3-O-glucoside-7-O-rhamnoside, quercetin 3-O-rutinoside, quercetin 3-O-glucoside, isorhamnetin 3-O-rutinoside, isorhamnetin 3-O-glucoside, quercetin, kaempferol 7-O-rhamnoside, kaempferol and isorhamnetin. Both correlation coefficient of similarity in chromatograms and relative peak areas of characteristic compounds were calculated for quantitative expression of the HPLC fingerprints. Our results revealed that the chromatographic fingerprint combining similarity evaluation could efficiently identify and distinguish sea buckthorn berries from different species. However, no obvious difference between RS and RY suggested that the two subspecies might have very close relationship in terms of chemotaxonomy. The established method was considered to be suitable for fingerprint analysis to check the genuine origin and control the quality of sea buckthorn berries and extracts.
采用高效液相色谱法（HPLC），建立了一种化学指纹图谱方法，用于研究和展示不同产地沙棘果实中黄酮类化合物的差异。共分析了34个样品，包括15个中国沙棘（Hippophae rhamnoides ssp. sinensis）样品、7个云南沙棘（H. rhamnoindes ssp. yunnanensis）样品、5个卧龙沙棘（H. rhamnoides ssp. wolongensis）样品、4个肋果沙棘（H. neurocarpa ssp. stellatopilosa）样品和3个西藏沙棘（H. tibetana）样品。在HPLC色谱图中，鉴定出12种黄酮类化合物，包括槲皮素3 - O - 槐糖苷 - 7 - 鼠李糖苷、山柰酚3 - O - 槐糖苷 - 7 - O - 鼠李糖苷、异鼠李素3 - O - 槐糖苷 - 7 - O - 鼠李糖苷、异鼠李素3 - O - 葡萄糖苷 - 7 - O - 鼠李糖苷、槲皮素3 - O - 芸香糖苷、槲皮素3 - O - 葡萄糖苷、异鼠李素3 - O - 芸香糖苷、异鼠李素3 - O - 葡萄糖苷、槲皮素、山柰酚7 - O - 鼠李糖苷、山柰酚和异鼠李素。计算了色谱图相似度的相关系数和特征化合物的相对峰面积，用于定量表达HPLC指纹图谱。结果表明，结合相似度评价的色谱指纹图谱能够有效鉴定和区分不同种的沙棘果实。然而，中国沙棘和云南沙棘之间没有明显差异，表明这两个亚种在化学分类学方面可能关系非常密切。所建立的方法被认为适用于指纹图谱分析，以检查沙棘果实和提取物的正宗来源并控制其质量。
