评估吡咯烷二硫代氨基甲酸盐对葡聚糖硫酸钠诱导的小鼠结肠炎炎症抑制作用。
Evaluation of the effect of pyrrolidine dithiocarbamate in suppressing inflammation in mice with dextran sodium sulfate-induced colitis.
作者信息
Hirata Ichiro, Yasumoto Shingo, Toshina Ken, Inoue Takuya, Nishikawa Takashi, Murano Naoko, Murano Mitsuyuki, Wang Fang-Yu, Katsu Ken-ichi
机构信息
Department of Gastroenterology, Fujita Health University, Toyoake, Aichi 470-1192, Japan.
出版信息
World J Gastroenterol. 2007 Mar 21;13(11):1666-71. doi: 10.3748/wjg.v13.i11.1666.
Abstract
AIM
To evaluate the effect of pyrrolidine dithio-carbamate (PDTC; an NF-kappaB inhibitor) administered at low (50 mg/kg) and high (100 mg/kg) doses in suppressing colitis in mice with dextran sodium sulfate (DSS)-induced colitis.
METHODS
Mice were divided into a DSS-untreated group (normal group), DSS-treated control group, DSS+PDTC-treated group I (low-dose group), and DSS+PDTC-treated group II (high-dose group). In each group, the disease activity index score (DAI score), intestinal length, histological score, and the levels of activated NF-kappaB and inflammatory cytokines (IL-1beta and TNF-alpha) in tissue were measured.
RESULTS
The DSS+PDTC-treated group II exhibited suppression of shortening of intestinal length and reduction of DAI score. Activated NF-kappaB level and IL-1beta and TNF-alpha levels were significantly lower in DSS+PDTC-treated group II.
CONCLUSION
These findings suggest that PDTC is useful for the treatment of ulcerative colitis.
摘要
目的
评估低剂量（50毫克/千克）和高剂量（100毫克/千克）的吡咯烷二硫代氨基甲酸盐（PDTC；一种核因子κB抑制剂）对葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎的抑制作用。
方法
将小鼠分为未用DSS处理的组（正常组）、用DSS处理的对照组、DSS + PDTC处理的I组（低剂量组）和DSS + PDTC处理的II组（高剂量组）。测量每组的疾病活动指数评分（DAI评分）、肠长度、组织学评分以及组织中活化的核因子κB和炎性细胞因子（IL - 1β和TNF - α）的水平。
结果
DSS + PDTC处理的II组表现出肠长度缩短受到抑制且DAI评分降低。DSS + PDTC处理的II组中活化的核因子κB水平以及IL - 1β和TNF - α水平显著更低。
结论
这些发现表明PDTC对溃疡性结肠炎的治疗有用。
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本文引用的文献
1
Plant sterol guggulsterone inhibits nuclear factor-kappaB signaling in intestinal epithelial cells by blocking IkappaB kinase and ameliorates acute murine colitis.
Inflamm Bowel Dis. 2006 Dec;12(12):1152-61. doi: 10.1097/01.mib.0000235830.94057.c6.
2
Pyrrolidine dithiocarbamate reduces ischemia-reperfusion injury of the small intestine.
World J Gastroenterol. 2005 Dec 14;11(46):7308-13. doi: 10.3748/wjg.v11.i46.7308.
3
Pyrrolidine dithiocarbamate reduces lung reperfusion injury.
J Surg Res. 2003 Jun 1;112(1):12-8. doi: 10.1016/s0022-4804(03)00139-2.
4
Pyrrolidinedithiocarbamate inhibits NF-kappaB activation and IL-8 production in intestinal epithelial cells.
Immunol Lett. 2003 Jan 2;85(1):41-6. doi: 10.1016/s0165-2478(02)00208-0.
5
Pyrrolidine dithiocarbamate protects against thioacetamide-induced fulminant hepatic failure in rats.
J Hepatol. 2002 Mar;36(3):370-7. doi: 10.1016/s0168-8278(01)00290-2.
6
Pyrrolidine dithiocarbamate attenuates the development of acute and chronic inflammation.
Br J Pharmacol. 2002 Jan;135(2):496-510. doi: 10.1038/sj.bjp.0704463.
7
IkappaBbeta, but not IkappaBalpha, functions as a classical cytoplasmic inhibitor of NF-kappaB dimers by masking both NF-kappaB nuclear localization sequences in resting cells.
J Biol Chem. 2001 Nov 30;276(48):45225-35. doi: 10.1074/jbc.M105865200. Epub 2001 Sep 24.
8
Therapeutic effect of intracolonically administered nuclear factor kappa B (p65) antisense oligonucleotide on mouse dextran sulphate sodium (DSS)-induced colitis.
Clin Exp Immunol. 2000 Apr;120(1):51-8. doi: 10.1046/j.1365-2249.2000.01183.x.
9
The IKK complex: an integrator of all signals that activate NF-kappaB?
Trends Cell Biol. 2000 Apr;10(4):129-33. doi: 10.1016/s0962-8924(00)01729-3.
10
NF-kappaB controls cell growth and differentiation through transcriptional regulation of cyclin D1.
Mol Cell Biol. 1999 Aug;19(8):5785-99. doi: 10.1128/MCB.19.8.5785.
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