Liu J, Li D B, Yang J C
Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.
Prikl Biokhim Mikrobiol. 2007 Mar-Apr;43(2):234-9.
The development of solid state fermentation (SSF) technology is very important to the production of cellulase and ultimately to the utilization of natural cellulose. However, inadequate dissipation of heat generated by biological activities has prevented solid state fermentation from large-scale applications. The paper deals with the development of a novel SSF bioreactor with air pressure pulsation. By developing a measurement and control system under Virtual Instrument (VI) concept, performance of SSF bioreactor with pressure pulsation was studied by cultivating Trichoderma koningii in solid medium made of wheat bran and corncob. The cooling effects of pressure pulsation on solid porous beds are discussed. Experimental results show that pressure pulsation enhances medium moisture evaporation, and hence, heat dissipation. Furthermore, through changing the pressure pulsation directions, it is able to mitigate the temperature gradients in the bioreactor. To sum up, pressure pulsation can provide the microbes with a growing environment at optimal temperature and medium water content.
固态发酵(SSF)技术的发展对于纤维素酶的生产以及最终天然纤维素的利用非常重要。然而,生物活动产生的热量散热不足阻碍了固态发酵的大规模应用。本文论述了一种新型的带气压脉动的固态发酵生物反应器的开发。通过在虚拟仪器(VI)概念下开发测量与控制系统,在由麦麸和玉米芯制成的固体培养基中培养康氏木霉,研究了带压力脉动的固态发酵生物反应器的性能。讨论了压力脉动对固体多孔床的冷却效果。实验结果表明,压力脉动增强了培养基水分蒸发,从而促进了散热。此外,通过改变压力脉动方向,能够减轻生物反应器中的温度梯度。总之,压力脉动可以为微生物提供一个温度和培养基含水量适宜的生长环境。