存活素表达在肝细胞癌患者中的临床病理意义

Clinicopathological significance of survivin expression in patients with hepatocellular carcinoma.

作者信息

Chau G-Y, Lee A F-Y, Tsay S-H, Ke Y-R, Kao H-L, Wong F-H, Tsou A-P, Chau Y-P

机构信息

Department of Surgery, Taipei Veterans General Hospital, Taipei, Taiwan, ROC.

出版信息

Histopathology. 2007 Aug;51(2):204-18. doi: 10.1111/j.1365-2559.2007.02738.x. Epub 2007 Jun 8.

DOI:10.1111/j.1365-2559.2007.02738.x

PMID:17559540

Abstract

AIMS

Survivin, a newly discovered member of the inhibitor of apoptosis protein family, is suggested to be involved in liver carcinogenesis. The aim was to investigate the clinical significance of survivin expression in resected hepatocellular carcinoma (HCC) and paired adjacent non-tumour tissue.

METHODS AND RESULTS

Immunohistochemistry, reverse transcriptase-polymerase chain reaction and Western blots were used to examine survivin mRNA and protein levels in 94 specimens of HCC tissues at different TNM stages and the data were correlated with the clinicopathological profiles. Patients were categorized into those with high tumour survivin protein levels (T-N >or= -1) and those with low levels (T-N < -1). Follow-up data were collected prospectively. mRNA levels of survivin and its splice variants in tumour tissue were significantly higher than in paired non-tumour tissue. However, survivin protein levels in paired non-tumour tissue were significantly higher than in tumour tissue from all three TNM stages. Additionally, high tumour survivin protein levels (T-N >or= -1) correlated with a better prognosis and low levels (T-N < -1) with a worse survival rate.

CONCLUSIONS

High cytoplasmic survivin protein levels in HCC tissues seem to be an indicator of better prognosis in HCC patients after resection.

摘要

目的

Survivin是新发现的凋亡抑制蛋白家族成员，提示其与肝癌发生有关。本研究旨在探讨Survivin在手术切除的肝细胞癌（HCC）及配对的癌旁非肿瘤组织中的表达及其临床意义。

方法与结果

采用免疫组化、逆转录聚合酶链反应及蛋白质免疫印迹法检测94例不同TNM分期的HCC组织标本中Survivin的mRNA和蛋白水平，并分析其与临床病理特征的相关性。将患者分为肿瘤Survivin蛋白水平高（T-N≥-1）和低（T-N＜-1）两组。前瞻性收集随访数据。肿瘤组织中Survivin及其剪接变异体的mRNA水平显著高于配对的非肿瘤组织。然而，配对非肿瘤组织中Survivin蛋白水平显著高于所有三个TNM分期的肿瘤组织。此外，肿瘤Survivin蛋白水平高（T-N≥-1）与较好的预后相关，而水平低（T-N＜-1）与较差的生存率相关。