成人初诊骨髓增生异常综合征（MDS）患者骨髓中凋亡调控基因表达的分析
Analysis of apoptosis regulatory genes expression in the bone marrow (BM) of adult de novo myelodysplastic syndromes (MDS).
作者信息
Economopoulou C, Pappa V, Kontsioti F, Papageorgiou S, Kapsimali V, Papasteriadi C, Economopoulou P, Papageorgiou E, Dervenoulas J, Economopoulos T
机构信息
Attikon University Hospital, 2nd Propedeutic Clinic of Internal., 1 Rimini St., Athens, Haidari, Greece.
出版信息
Leuk Res. 2008 Jan;32(1):61-9. doi: 10.1016/j.leukres.2007.04.012. Epub 2007 Jun 26.
The aim of the present study was to examine caspases, granzyme B and bcl-2 family mRNA expression and the degree of apoptosis in the bone marrow (BM) of 46 Myelodysplastic Syndromes (MDS) and to correlate our findings with clinical parameters. The degree of apoptosis was determined by Annexin V, whereas expression of genes was determined using a multiprobe RNase Protection System. A positive correlation was found between caspases 8, 5, 3, 2, 1 and the level of apoptosis. bfl1 and mcl1 levels were significantly higher in patients with BM blasts >5%. Cases with ratio of bid expression >1 compared to normal pool were associated with IPSS values < or =1.
本研究的目的是检测46例骨髓增生异常综合征（MDS）患者骨髓中半胱天冬酶、颗粒酶B和bcl-2家族mRNA的表达以及凋亡程度，并将我们的研究结果与临床参数相关联。凋亡程度通过膜联蛋白V测定，而基因表达则使用多探针核糖核酸酶保护系统进行测定。发现半胱天冬酶8、5、3、2、1与凋亡水平呈正相关。骨髓原始细胞>5%的患者bfl1和mcl1水平显著更高。与正常样本相比，bid表达比值>1的病例与国际预后评分系统（IPSS）值≤1相关。
Zotero 插件