通过多重金纳米棒探针进行细胞表面标志物的身份分析。

Identity profiling of cell surface markers by multiplex gold nanorod probes.

作者信息

Yu Chenxu, Nakshatri Harikrishna, Irudayaraj Joseph

机构信息

Department of Agricultural and Biological Engineering and Bindley Biosciences Center, Purdue University, 225 South University Drive, West Lafayette, Indiana 47906, USA.

出版信息

Nano Lett. 2007 Aug;7(8):2300-6. doi: 10.1021/nl070894m. Epub 2007 Jun 29.

DOI:10.1021/nl070894m

PMID:17602538

Abstract

Gold nanorod molecular probes (GNrMPs) were designed and fabricated for multiplex identification of cell surface markers in HBECs. Cells were probed directly using dark field microscopy integrated with a spectral imager for simultaneous detection of up to three surface markers. The immunophenotype composition of these cell lines indicative of their metastasis potential was assessed using the GNrMPs. The technique has the potential to become an important tool for diagnosis and prognosis of breast and other cancers.

摘要

设计并制造了金纳米棒分子探针（GNrMPs）用于多重识别支气管上皮细胞（HBECs）的细胞表面标志物。使用与光谱成像仪集成的暗场显微镜直接探测细胞，以同时检测多达三种表面标志物。使用GNrMPs评估了这些具有转移潜能的细胞系的免疫表型组成。该技术有可能成为乳腺癌和其他癌症诊断及预后的重要工具。