布氏锥虫29-13株在采采蝇载体中可诱导但不允许其繁殖。
Trypanosoma brucei 29-13 strain is inducible in but not permissive for the tsetse fly vector.
作者信息
Herder Stéphane, Votýpka Jan, Jirků Milan, Rádrová Jana, Janzen Christian J, Lukes Julius
机构信息
UMR 177 IRD CIRAD, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France.
出版信息
Exp Parasitol. 2007 Sep;117(1):111-4. doi: 10.1016/j.exppara.2007.05.011. Epub 2007 Jun 3.
Using green fluorescent protein as a reporter, we have shown that the strain 29-13 of Trypanosoma brucei, widely used for inducible down-regulation of mRNA, is inducible in, but not permissive for the tsetse flies Glossina palpalis gambiensis and Glossina morsitans morsitans. Within two weeks post-infection, 42% males and females of teneral and non-teneral tsetse flies harboured intestinal infections, yet not a single infection progressed into the salivary glands.
利用绿色荧光蛋白作为报告基因，我们发现广泛用于mRNA诱导下调的布氏锥虫29-13株，在冈比亚采采蝇和莫氏采采蝇体内可诱导，但不具有感染性。感染后两周内，42%的新羽化和非新羽化的采采蝇雌雄个体肠道受到感染，但没有一例感染发展到唾液腺。
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