[鸡源免疫单链抗体文库的构建与鉴定]
[Construction and identification of an immune single chain antibody library derived from a chicken].
作者信息
Chen Hong-bing
机构信息
Key Laboratory of Food Science of Ministry of Education, Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007 Jul;23(7):645-8.
AIM
To develop a method for constructing an immune single chain antibody library derived from a chicken.
METHODS
According to the principle of phage display and the character of chicken antibody genes, an immune single chain antibody library was constructed by using the spleen B cells which were extracted from a chicken immunized with streptococcus mutans as antibody gene sources. According to the principle of immobilized panning the specific chicken single chain phage antibody was selected from the single chain antibody library to identify the immune antibody library.
RESULTS
The repertoire of the chicken heavy-chain and light-chain variable regions was obtained from the total intact RNA of chicken spleen. The phage antibody library was generated and its size was determined to be 1.6x10(7). A specific chicken single chain phage antibody against streptococcus mutans was selected and its DNA was sequenced.
CONCLUSION
An immune single chain antibody library derived from a chicken can be constructed successfully based on the principle of phage antibody library and the character of chicken antibody genes.
目的
建立一种构建鸡源免疫单链抗体文库的方法。
方法
根据噬菌体展示原理及鸡抗体基因特点，以经变形链球菌免疫的鸡脾脏B细胞为抗体基因来源构建免疫单链抗体文库。依据固定化筛选原理，从单链抗体文库中筛选特异性鸡单链噬菌体抗体以鉴定免疫抗体文库。
结果
从鸡脾脏总完整RNA中获得鸡重链和轻链可变区文库。构建了噬菌体抗体文库，其库容量为1.6×10⁷。筛选出一株抗变形链球菌的特异性鸡单链噬菌体抗体并对其DNA进行测序。
结论
基于噬菌体抗体文库原理及鸡抗体基因特点可成功构建鸡源免疫单链抗体文库。
Zotero 插件