白色念珠菌分泌天冬氨酸蛋白酶在急性阴道念珠菌病中的表达

Expression of Candida albicans secreted aspartyl proteinase in acute vaginal candidiasis.

作者信息

Lin Nengxing, Feng Jing, Tu Yating, Feng Aiping

机构信息

Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. Linnengxing@hotmail-com

出版信息

J Huazhong Univ Sci Technolog Med Sci. 2007 Jun;27(3):333-5. doi: 10.1007/s11596-007-0330-8.

DOI:10.1007/s11596-007-0330-8

PMID:17641856

Abstract

In order to analyze the in vivo expression of Candida albicans secreted aspartyl proteinases (SAP) in human vaginal infection, the vaginal secretion from 29 human subjects was collected by vaginal swab, and the expression of SAP1-SAP6 was detected by reverse-transcriptase polymerase chain reaction using specific primer sets. It was found that Sap2 and Sap5 were the most common genes expressed during infection; Sap3 and Sap4 were detected in all subjects and all 6 SAP genes were simultaneously expressed in some patients with vaginal candidiasis. It was suggested that the SAP family is expressed by Candida albicans during infection in human and that Candida albicans infection is associated with the differential expression of individual SAP genes which may be involved in the pathogenesis of vaginal candidiasis.

摘要

为分析白色念珠菌分泌天冬氨酸蛋白酶（SAP）在人类阴道感染中的体内表达情况，通过阴道拭子收集了29名受试者的阴道分泌物，并使用特异性引物对通过逆转录聚合酶链反应检测了SAP1 - SAP6的表达。结果发现，Sap2和Sap5是感染期间最常表达的基因；在所有受试者中均检测到Sap3和Sap4，并且在一些阴道念珠菌病患者中同时表达了所有6种SAP基因。这表明白色念珠菌在人类感染期间表达SAP家族，并且白色念珠菌感染与个别SAP基因的差异表达有关，这些基因可能参与阴道念珠菌病的发病机制。