Osteoinduction in human fat-derived stem cells by recombinant human bone morphogenetic protein-2 produced in Escherichia coli.

作者信息

Bessa Paulo C, Pedro A J, Klösch B, Nobre A, van Griensven M, Reis R L, Casal M

机构信息

3B's Research Group - Biomaterials, Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.

出版信息

Biotechnol Lett. 2008 Jan;30(1):15-21. doi: 10.1007/s10529-007-9501-8. Epub 2007 Aug 3.

DOI:10.1007/s10529-007-9501-8

PMID:17673947

Abstract

Bioactive recombinant human bone morphogenetic protein-2 (rhBMP-2) was obtained using Escherichia coli pET-25b expression system: 55 mg purified rhBMP-2 were achieved per g cell dry wt, with up to 95% purity. In murine C2C12 cell line, rhBMP-2 induced an increase in the transcription of Smads and of osteogenic markers Runx2/Cbfa1 and Osterix, measured by semi-quantitative RT-PCR. Bioassays performed in human fat-derived stem cells showed an increased activity of the early osteogenic marker, alkaline phosphatase, and the absence of cytotoxicity.

摘要