Karidi Konstantina, Reedijk Jan, Hadjiliadis Nick, Garoufis Achilleas
Laboratory of Inorganic and General Chemistry, Department of Chemistry, University of Ioannina, Ioannina 45110, Greece.
J Inorg Biochem. 2007 Oct;101(10):1483-91. doi: 10.1016/j.jinorgbio.2007.06.027. Epub 2007 Jun 27.
The DNA-binding properties of a number of ruthenium oligopyridine complexes with conjugated amino acids having the general formulae Ru(terpy)(4-COY-4'-Mebpy)(X)(+), X=NO (n=3), X=Cl (n=1) and NO(2) (n=1) and Y=AlaCONH(2) and TrpCONH(2) are reported. The new complexes were spectroscopically characterized and their DNA-binding properties were studied by means of circular dichroism (CD), (23)Na and (31)P NMR spectroscopy. The results show that the chlorido complexes interact by coordination to the DNA bases with the conjugated amino acid able to provide an additional interaction with the DNA helix. In addition, electrostatic interactions between all studied complexes and the DNA polyanion were observed. The nitro complexes were found to be insignificant, affecting only the (31)P NMR signal, probably due to changes in the hydration sphere of the DNA close to the phosphates.
报道了一些通式为Ru(terpy)(4-COY-4'-Mebpy)(X)(+)的钌联吡啶配合物与共轭氨基酸的DNA结合特性，其中X = NO (n = 3)、X = Cl (n = 1) 和NO₂ (n = 1)，Y = AlaCONH₂和TrpCONH₂。通过光谱对新配合物进行了表征，并利用圆二色性（CD）、²³Na和³¹P核磁共振光谱研究了它们的DNA结合特性。结果表明，氯配合物通过与DNA碱基配位相互作用，共轭氨基酸能够与DNA螺旋提供额外的相互作用。此外，观察到所有研究的配合物与DNA聚阴离子之间的静电相互作用。发现硝基配合物的影响不显著，仅影响³¹P核磁共振信号，这可能是由于靠近磷酸盐的DNA水合球发生了变化。
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