乙胺嗪给药后小鼠睾丸间质细胞超微结构及睾酮分泌的变化

Changes in mouse Leydig cells ultrastructure and testosterone secretion after diethylcarbamazine administration.

作者信息

Saraiva Karina Lidianne Alcântara, Silva Valdemiro Amaro Da, Torres Dilênia De Oliveira Cipriano, Donato Mariana Aragão Matos, Peres Newton Gil, Souza José Roberto Botelho De, Peixoto Christina Alves

机构信息

Departamento de Biologia Celular e Ultraestrutura, Centro de Pesquisas Aggeu Magalhães (FIOCRUZ), e Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, Recife, Brazil.

出版信息

Micron. 2008 Jul;39(5):580-6. doi: 10.1016/j.micron.2007.06.003. Epub 2007 Jun 24.

DOI:10.1016/j.micron.2007.06.003

PMID:17681769

Abstract

Diethylcarbamazine (DEC) has been proven to be highly effective against lymphatic filariasis, although its effect on vertebrate cells remains uncertain. Mice Leydig cells after treatment with 200mg/kg of DEC for 12 days showed numerous lipid droplets, degenerated mitochondria, residual bodies and several giant whorl-like smooth endoplasmic reticulum, some of them encircling large lipids droplets. Treatment with lower dosages showed similar alterations on Leydig cells and the morphological effects decreased directly proportional to the drug concentration. Serum testosterone levels were significantly lower only in 200 mg/kg DEC-treated group when compared to the controls. However, no significant changes were observed in the pregnancy rates and offspring number of DEC-treated male-mated female mice in any doses studied. The results obtained in the present study are consistent with the hypothesis that DEC has some effects on mice Leydig cells, although they were not sufficient enough to interfere with the rodent fertility.

摘要

乙胺嗪（DEC）已被证明对淋巴丝虫病非常有效，尽管其对脊椎动物细胞的影响仍不确定。用200mg/kg的DEC处理12天后的小鼠睾丸间质细胞显示出大量脂滴、退化的线粒体、残余小体和几个巨大的涡状滑面内质网，其中一些围绕着大的脂滴。较低剂量处理对睾丸间质细胞显示出类似的改变，并且形态学效应与药物浓度成反比降低。与对照组相比，仅在200mg/kg DEC处理组中血清睾酮水平显著降低。然而，在任何研究剂量下，用DEC处理的雄性与雌性小鼠交配后的妊娠率和后代数量均未观察到显著变化。本研究获得的结果与DEC对小鼠睾丸间质细胞有一些影响的假设一致，尽管这些影响不足以干扰啮齿动物的生育能力。