基于固定在聚乙烯醇缩甲醛中的乳糖酶和半乳糖氧化酶的乳糖生物传感器。

Lactose biosensor based on lactase and galactose oxidase immobilized in polyvinyl formal.

作者信息

Sharma Sandeep K, Kumar Ashok, Chaudhary Reeti, Pundir C S, Sehgal Neeta

机构信息

Institute of Genomics and Integrative Biology, Delhi, India.

出版信息

Artif Cells Blood Substit Immobil Biotechnol. 2007;35(4):421-30. doi: 10.1080/10731190701460309.

DOI:10.1080/10731190701460309

PMID:17701488

Abstract

A lactose biosensor was developed by immobilizing lactase and galactose oxidase in a polyvinyl formal membrane and was attached to the oxygen electrode of a dissolved oxygen analyzer for estimation of lactose in milk and food products. The enzyme immobilized polyvinyl formal membrane was characterized by atomic force microscopy. The biosensor showed the linearity for 1-7 g dl(-1) of lactose and can be reused for up to 20 measurements. The effects of pH, temperature and the stability of the immobilized lactase and galactose oxidase in PVF membrane were also studied. The enzyme membrane was found stable up to 35 degrees C and had a shelf-life of more than three months at 4 degrees C.

摘要

通过将乳糖酶和半乳糖氧化酶固定在聚乙烯醇缩甲醛膜中制备了一种乳糖生物传感器，并将其连接到溶解氧分析仪的氧电极上，用于测定牛奶和食品中的乳糖。采用原子力显微镜对固定化酶的聚乙烯醇缩甲醛膜进行了表征。该生物传感器对1-7 g dl(-1)的乳糖具有线性响应，可重复使用多达20次。还研究了pH值、温度以及固定化乳糖酶和半乳糖氧化酶在PVF膜中的稳定性。发现酶膜在35℃以下稳定，在4℃下保质期超过三个月。