变应性鼻炎中蛋白酶激活受体2的上调
Up-regulation of protease-activated receptor 2 in allergic rhinitis.
作者信息
Lee Heung-Man, Kim Hyo Yeol, Kang Hee Joon, Woo Jeong Soo, Chae Sung Won, Lee Sang Hag, Hwang Soon Jae
机构信息
Department of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, Korea.
出版信息
Ann Otol Rhinol Laryngol. 2007 Jul;116(7):554-8. doi: 10.1177/000348940711600712.
OBJECTIVES
We compared the patterns of PAR-2 messenger RNA (mRNA) and protein expression in the nasal mucosa of subjects with and without allergic rhinitis.
METHODS
Biopsy specimens were obtained from 10 patients with allergic rhinitis and 10 normal controls. RNA was extracted from the nasal mucosa, and semiquantitative reverse transcription-polymerase chain reaction was performed for PAR-2. Tissue sections were immunostained for PAR-2 by use of specific antibody.
RESULTS
The expression levels of PAR-2 mRNA in allergic rhinitis nasal mucosa were significantly up-regulated as compared with those in normal nasal mucosa. PAR-2 immunoreactivity was observed in the epithelium and submucosal glands in both normal controls and subjects with allergic rhinitis. Stronger immunoreactivity for PAR-2 was observed in allergic rhinitis nasal mucosa as compared with normal nasal mucosa.
CONCLUSIONS
These results suggest that PAR-2 may be involved in allergic nasal inflammation.
目的
我们比较了患有和未患过敏性鼻炎的受试者鼻黏膜中PAR-2信使核糖核酸（mRNA）和蛋白表达模式。
方法
从10例过敏性鼻炎患者和10例正常对照者获取活检标本。从鼻黏膜提取RNA，并对PAR-2进行半定量逆转录聚合酶链反应。使用特异性抗体对组织切片进行PAR-2免疫染色。
结果
与正常鼻黏膜相比，过敏性鼻炎鼻黏膜中PAR-2 mRNA的表达水平显著上调。在正常对照者和过敏性鼻炎患者的上皮及黏膜下腺体中均观察到PAR-2免疫反应性。与正常鼻黏膜相比，在过敏性鼻炎鼻黏膜中观察到更强的PAR-2免疫反应性。
结论
这些结果表明PAR-2可能参与过敏性鼻炎症。
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