产气荚膜梭菌的一种小的酸溶性芽孢蛋白对枯草芽孢杆菌芽孢抗性特性的影响。
Effect of a small, acid-soluble spore protein from Clostridium perfringens on the resistance properties of Bacillus subtilis spores.
作者信息
Leyva-Illades Juan Francisco, Setlow Barbara, Sarker Mahfuzur R, Setlow Peter
机构信息
Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030-3305, USA.
出版信息
J Bacteriol. 2007 Nov;189(21):7927-31. doi: 10.1128/JB.01179-07. Epub 2007 Aug 31.
Abstract
Alpha/beta-type small, acid-soluble spore proteins (SASP) are essential for the resistance of DNA in spores of Bacillus species to damage. An alpha/beta-type SASP, Ssp2, from Clostridium perfringens was expressed at significant levels in B. subtilis spores lacking one or both major alpha/beta-type SASP (alpha- and alpha- beta- strains, respectively). Ssp2 restored some of the resistance of alpha- beta- spores to UV and nitrous acid and of alpha- spores to dry heat. Ssp2 also restored much of the resistance of alpha- spores to nitrous acid and restored full resistance of alpha- spores to UV and moist heat. These results further indicate the interchangeability of alpha/beta-type SASP in DNA protection in spores.
摘要
α/β型小酸溶性芽孢蛋白（SASP）对于芽孢杆菌属芽孢中的DNA抗损伤能力至关重要。来自产气荚膜梭菌的一种α/β型SASP，即Ssp2，在缺乏一种或两种主要α/β型SASP的枯草芽孢杆菌芽孢（分别为α-菌株和α-β-菌株）中大量表达。Ssp2恢复了α-β-芽孢对紫外线和亚硝酸的部分抗性，以及α-芽孢对干热的抗性。Ssp2还恢复了α-芽孢对亚硝酸的大部分抗性，并恢复了α-芽孢对紫外线和湿热的完全抗性。这些结果进一步表明了α/β型SASP在芽孢DNA保护中的可互换性。
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