GAC基因产物对酿酒酵母中乙二醛酶I基因mRNA水平的积极作用。

Positive effect of GAC gene product on the mRNA level of glyoxalase I gene in Saccharomyces cerevisiae.

作者信息

Inoue Y, Yano H, Ginya H, Tsuchiyama H, Murata K, Kimura A

机构信息

Research Institute for Food Science, Kyoto University, Japan.

出版信息

Biotechnol Appl Biochem. 1991 Dec;14(3):391-4.

PMID:1777125

Abstract

A DNA fragment carrying a part of the structural gene for yeast (Saccharomyces cerevisiae) glyoxalase I was cloned from a lambda gt11 expression library using anti-glyoxalase IIgG as a probe. By Northern blotting analysis, the amount of glyoxalase I mRNA was found to increase in yeast cells containing plasmids carrying the GAC gene, which is a positive regulator for yeast glyoxalase I activity. This suggests that the GAC gene product may accelerate the transcription of glyoxalase I gene or may have some positive effects on the accumulation of glyoxalase I mRNA in yeast cells.

摘要

利用抗乙二醛酶II IgG作为探针，从λgt11表达文库中克隆出一段携带酵母（酿酒酵母）乙二醛酶I部分结构基因的DNA片段。通过Northern印迹分析发现，在含有携带GAC基因质粒的酵母细胞中，乙二醛酶I mRNA的量增加，GAC基因是酵母乙二醛酶I活性的正调控因子。这表明GAC基因产物可能加速乙二醛酶I基因的转录，或者对酵母细胞中乙二醛酶I mRNA的积累有一些积极作用。