ABO blood grouping of semen from mixed body fluids with monoclonal antibody to tissue-specific epitopes on seminal ABO blood group substance.

Practical methods were developed for ABO blood grouping of semen from mixed body fluids. An monoclonal antibody (P6-5H) which recognizes a tissue-specific epitope on a seminal ABO blood group substance (alpha 2-seminoglycoprotein) was used as a solid phase antibody for selective capture of the seminal ABO blood group substance. ABO blood group epitopes of secretor and non-secretor semen were detected in dilutions of 8 x 10(3)-3.2 x 10(4) and 8 x 10(3)-fold, respectively, by sandwich ELISA. ABO blood group epitopes were also detected in dilutions up to 4 x 10(3)-fold, irrespective of secretor status, by the sandwich absorption-elution test.