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食用蘑菇佛罗里达侧耳水溶性葡聚糖(Fr.I)的结构分析

Structural analysis of a water-soluble glucan (Fr.I) of an edible mushroom, Pleurotus sajor-caju.

作者信息

Pramanik Malay, Chakraborty Indranil, Mondal Soumitra, Islam Syed S

机构信息

Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721 102, West Bengal, India.

出版信息

Carbohydr Res. 2007 Dec 10;342(17):2670-5. doi: 10.1016/j.carres.2007.08.012. Epub 2007 Aug 23.

Abstract

A water-soluble glucan was obtained from the fruit bodies of an edible mushroom, Pleurotus sajor-caju, by hot water extraction, ethanol precipitation, dialysis, and Sepharose 6B gel filtration. On the basis of total hydrolysis, methylation analysis, periodate oxidation, and NMR studies ((1)H, (13)C, DQF-COSY, TOCSY, NOESY, and HSQC), the structure of the repeating unit of the glucan is determined as [carbohydrate structure: see text].

摘要

通过热水提取、乙醇沉淀、透析和琼脂糖6B凝胶过滤,从食用蘑菇佛罗里达侧耳的子实体中获得了一种水溶性葡聚糖。基于完全水解、甲基化分析、高碘酸盐氧化和核磁共振研究(氢谱、碳谱、双量子滤波相关谱、全相关谱、核Overhauser效应谱和异核单量子相干谱),确定了该葡聚糖重复单元的结构为[碳水化合物结构:见正文]。

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