Iqbal M P, Hussain R
Department of Biochemistry, Aga Khan University Medical College, Karachi, Pakistan.
Ital J Biochem. 1991 Jul-Aug;40(4):207-15.
A competitive enzyme-linked immunosorbent assay (ELISA) has been developed for the enzyme, dihydrofolate reductase. The sensitivity of the assay system approximates 3 ng of immunoreactive enzyme protein which is comparable to the sensitivity achieved in the radioimmunoassay (RIA) for this enzyme. The mean coefficient of variation for within--and between--assay precision was less than 13%. The assay appears to be specific and valid as the concentration of the enzyme is the same whether measured by ELISA or [3H]-methotrexate binding. Since this method, like the RIA, measures the mass concentration of enzyme protein, in conjunction with a [3H]-methotrexate binding assay it will be useful for quantitating functional as well as non-functional immunoreactive forms of the enzyme.
已开发出一种针对二氢叶酸还原酶的竞争性酶联免疫吸附测定（ELISA）方法。该测定系统的灵敏度约为3纳克免疫反应性酶蛋白，这与该酶放射免疫测定（RIA）所达到的灵敏度相当。测定内和测定间精密度的平均变异系数小于13%。该测定似乎具有特异性且有效，因为无论是通过ELISA还是[3H] - 甲氨蝶呤结合法测量，酶的浓度都是相同的。由于这种方法与RIA一样，测量的是酶蛋白的质量浓度，因此结合[3H] - 甲氨蝶呤结合测定，它将有助于定量该酶的功能性和非功能性免疫反应形式。
