Díaz C, Schilardi P L, Salvarezza R C, de Mele M Fernández Lorenzo
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Facultad de Ciencias Exactas, Universidad Nacional de La Plata-CONICET, Sucursal 4 Casilla de Correo 16, (1900) La Plata, Argentina.
Langmuir. 2007 Oct 23;23(22):11206-10. doi: 10.1021/la700650q. Epub 2007 Sep 20.
The adhesion of Pseudomonas fluorescens was studied on nano/microengineered surfaces. Results show that these bacteria formed well-defined aggregates on randomly oriented nanosized granular gold substrates. These aggregates consist of aligned ensembles of bacteria, with some of them strongly elongated. This kind of biological structure was not found on ordered engineered surfaces because bacterial alignment and cell-to-cell sticking were hindered. Importantly, differences in cell morphology, length, orientation, and flagellation were observed between bacteria attached on the ordered nano/microstructures and the randomly ordered surfaces. The implications of the results are related to the design of engineered surfaces to enhance (nanostructured filters) or inhibit (medical implants and industrial biofouling) bacterial colonization on the surfaces and to the biocontrol of soil ecosystems.
研究了荧光假单胞菌在纳米/微工程表面的黏附情况。结果表明，这些细菌在随机取向的纳米级颗粒金基底上形成了界限分明的聚集体。这些聚集体由排列整齐的细菌群体组成，其中一些细菌强烈伸长。在有序工程表面未发现这种生物结构，因为细菌的排列和细胞间黏附受到了阻碍。重要的是，观察到附着在有序纳米/微结构表面和随机有序表面上的细菌在细胞形态、长度、取向和鞭毛形成方面存在差异。这些结果的意义涉及到工程表面的设计，以增强（纳米结构过滤器）或抑制（医疗植入物和工业生物污垢）细菌在表面的定殖，以及土壤生态系统的生物控制。
